T cell reactivity against SARS-CoV-2 antigens analyzed by FluoroSpot

The SARS-COV-2 FluoroSpot results are visualized as Tukey box plots of serologically negative (n = 48) and positive donors (n = 100).

Stimulation indexes (Sls; spot count antigen stimulated sample divided by spot count unstimulated sample) of IFN-y, IL-2 and IFN-y + IL-2 T cell response against SARS-CoV-2 antigens indicate a highly significant difference (**; P value <0.0001) between both groups. P values are calculated by the unpaired, parametric t-test.

The solid line indicates the median, the box indicates the interquartile range (the difference between the 25th and 75th percentiles), the whiskers indicate the range excluding “outliers” and the points indicate outliers, points more than 1.5 IQR from the box.